Table 1.
Primer sequences used for quantitative real-time polymerase chain reaction.
| Genea | Primer sequence (5′–3′) | GenBank accession no. |
|---|---|---|
| IL-1β | GAAGTCAAGACCAAAGTGG (forward) TGAAGTCAACTATGTCCCG (reverse) |
M98820 |
| IL-6 | AAGTTTCTCTCCGCAAGAGACTTCCAG (forward) AGGCAAATTTCCTGGTTATATCCAGTT (reverse) |
NC005013 |
| TNF-α | CTTCAAGGGACAAGGCTG (forward) GAGGCTGACTTTCTCCTG (reverse) |
D00475 |
| MCP-1 | TAGCATCCACGTGCTGTCTC (forward) CCGACTCATTGGGATCATCT (reverse) |
NM031530 |
| RANTES | GCCAACCCAGAGAAGAAGTG (forward) ATCCCCAGCTGGTTAGGACT (reverse) |
NM031116 |
| GAPDH | TGCCAAGTATGATGACATCAAGAAG (forward) AGCCCAGGATGCCCTTTAGT (reverse) |
NM01008 |
a
TNF-α, tumor necrosis factor alpha; MCP-1, monocyte chemotactic protein-1; IL-1β, interleukin-1 beta; IL-6, interleukin-6; RANTES, regulated on activation, normal T expressed and secreted; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.