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. Author manuscript; available in PMC: 2013 Oct 1.
Published in final edited form as: Alcohol Clin Exp Res. 2012 Apr 6;36(10):1678–1687. doi: 10.1111/j.1530-0277.2012.01792.x

Figure 4. NMDA Receptor Subunits are Upregulated After Chronic Alcohol Exposure in Neural Cells Derived from Alcoholics.

Figure 4

Neural cells (age 12–15 weeks) derived from 7 different subjects, 3 non-alcoholics and 4 alcoholics, were treated daily with 50 mM alcohol for 7 days and levels of GRIN1, GRIN2A, GRIN2B, and GRIN2D mRNA expression was analyzed using RT-PCR. No significant changes were observed in the expression of these NMDA receptor subunit genes after 7-day alcohol exposure (relative to the sham condition) in neural cells derived from the group of non-alcoholic donor subjects (left group of data panels A–D). In contrast significant increases in mRNA levels compared to the sham condition were seen for neural cells derived from the alcoholic subject group for NMDA subunit genes (A) GRIN1, (B) GRIN2A, and (D) GRIN2D, while a trend towards significance was observed for (C) GRIN2B (p=0.06). Scatter plot symbols represent results from individual culture wells together with mean and SEM (significance level compared with sham condition * < 0.05, ** <0.01, *** <0.001).