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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: J Immunol. 2012 Jul 27;189(5):2512–2520. doi: 10.4049/jimmunol.1102892

Figure 2. DC Regulation Requires Cell Contact.

Figure 2

The experiment was designed as outlined in figure 1, and 96 well Transwell plates were used in this experiment. Each well contained complete RPMI medium (200 ul/well). LPMC were mixed with splenic OT2 T cells (ratio 2:1) and placed in the outer chamber (2×105 cells/well). In some cases, the outer chamber also contained DC (4×104 cells/well) derived from TI of Rag mice infected with Hpb for 2 wks (Hpb DC). Still other wells contained Hpb DC placed in the inner chamber with the LPMC + OT2 T cells in the outer chamber separated by a 0.4 um semi-permeable membrane. Data are mean ± SE of 3 independent experiments. *LPMC±OVA; or LPMC±OVA cultured without contact with Hpb DC, p<0.01.