Figure 5. DC Transfer Does not Diminish the Number of OT2 T Cells that Accumulate in the Gut or MLN.

Rag mice received 4×10⁶ splenic IL10 KO T cells displaying CD45.2 and 2×10⁶ OT2 T cells expressing CD45.1 given by i.p injection. Some mice also received DC (CD11c) (4×10⁴/mouse) from the MLN of Rag mice previously infected with Hpb, while other animals did not receive DC (No CD11c). At the end of the experiment, LPMC were isolated from the TI of either the control or CD11c transfer group. Also studied were dispersed MLN cells. Cells in the lymphoid gate were subject to flow analysis to determine the relative number of LP T cells expressing CD45.1. For each group, flow analysis was performed on pooled LPMC or MLN cells isolated from 4 individual mice. Data are representative of 3 independent experiments.