Table I.

Intestinal OT2 T cells rendered inert by DC transfer retain the capacity to respond to OVA

	Hpb OT2 + Non-T	Hpb OT2 + Hpb Non-T	OT2 + Non-T	OT2 + Hpb Non-T
IFNg
Cells	BD*	BD	BD*	BD
Cells +OVA	93±5	BD	72±10	BD
IL17
Cells	67±5*	70±6	67±10*	73±9
Cells +OVA	167±26	82±4	142±22	80±6

Intestinal OT2 T cells isolated from Rag mice who received DC from Hpb-infected animals (Hp OT2) respond to OVA when cultured with T cell-depleted LPMC (Non-T) from Rag mice who did not receive DC. Conversely, the OT2 cells fail to respond to antigen when they are cultured with their own LPMC (Hp OT2 + Hp Non-T). Moreover, intestinal OT2 T cells that normally respond to OVA (OT2 + Non-T) will fail to do so if they are cultured with Hpb Non-T.

The experimental design is outlined in figure 4, panel A, except that the recipient mice received CD45.1⁺ OT2 T cells to allow OT2 T cell isolation via FACS. Some mice also received DC from the MLN of Rag mice infected with Hpb for 2 wks (Hpb DC), whereas other mice received no DC. At the time of sacrifice, OT2 T cells were isolated from dispersed LPMC using FACS and anti-CD45.1 mAb. LPMC deleted of T cells (Non-T) were obtained using FACS and anti-Thy 1.2 mAb. OT2 T cells (10⁶) from the TI of either the Hpb DC recipients or control mice (No DC) were mixed with 2×10⁶ Non-T from the TI of either experimental group (Hpb non-T or non-T, no DC) and cultured without or with OVA (10 ug/ml) for 48h. Data are mean pg/ml±SE of three independent determinations.

^*cytokine vs. cytokine + OVA, p<0.01