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. Author manuscript; available in PMC: 2012 Sep 1.
Published in final edited form as: Thromb Res. 2012 Jun 9;130(3):e163–e170. doi: 10.1016/j.thromres.2012.05.021

Figure 1. Ixolaris blocks murine and human TF procoagulant activity to a similar extent.

Figure 1

The inhibitory effect of ixolaris on the TF activity of U87-MG human glioblastoma and B16F10 murine melanoma cell lines was tested by examining activation of FX by FVIIa in the presence of the cells. U87-MG and B16F10 cells were incubated with FVIIa [murine (mFVIIa) or human (hFVIIa) origin] for 10 min prior to the addition of FX [murine (mFX) or human (hFX) origin], either pre-incubated or not with varying amounts of ixolaris (0-5 nM). The amount of FXa formed was measured after 30 min of reaction time. Results are expressed as the percentage of FXa activation relative to the control sample not incubated with ixolaris (0 nM). Each data point represents the mean ± SD of replicates from three independent experiments. The assay conditions are described in the Methods section.