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Tmevpg1 is necessary but not sufficient for IFN-γ expression by Th1 cells. CMV-Tmevpg1 or CMV-empty expression plasmids were introduced into polarized cultures by nucleofection and cultures were restimulated with PMA and ionomycin. A, Tmevpg1 transcript levels are expressed relative to Gapdh. B, IFN-γ levels in Th0, Th1, and Th2 polarized cultures were determined by ELISA. Results represent the mean of at least three separate experiments
