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. Author manuscript; available in PMC: 2013 Aug 1.
Published in final edited form as: Curr Opin Chem Biol. 2012 May 25;16(3-4):292–299. doi: 10.1016/j.cbpa.2012.04.010

Figure 3.

Figure 3

(A) Schematic representation of the photocaged T7RNAP system. The photocaging group is represented by a blue sphere. NTPs are blocked from entering the active site of T7RNAP when the enzyme is caged. After UV irradiation the caging group is removed, NTPs are free to enter the active site and transcription is activated. (B) Photoactivation of EGFP expression in E. coli through light-activation of a photocaged T7RNAP; EGFP expression is restored after 10 minutes exposure to localized 365 nm irradiation. (C) Light-activation of T7RNAP activity and gene expression in vivo. (D) Light-activated MAP kinase signaling pathway. The photocaging group is represented by a blue sphere. (E) Light-activated translocation of EGFP-tagged ERK into the nucleus of HEK293 cells. Adapted with permission from Wiley-VCH Verlag GmbH & Co, © 2010, from [37] and from the American Chemical Society, © 2011, from [36].