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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Gene Ther. 2012 May 3;20(3):274–282. doi: 10.1038/gt.2012.38

Figure 7. Sizes of dystrophin gene RNA transcript in the various myocardial segments.

Figure 7

Sample from “Normal dog” demonstrate the predicted RNA transcript size band at 911 base-pair (bp) whereas an “Untreated GRMD dog” (natural occurrence of exon 7 skipping) produce a typical band at 792bp. The predicted size of bands for the in-frame skipping of treated dog with U7-ESE6/8 construct is 437bp for skipping of exons 6 to 8 and 305bp for skipping of exons 6 to 9. In the presented representative gel, two dominant new bands appear in samples 2, 4, 5, 8–12 and 16 of the treated dog. The lower band (300bp) corresponds to an in-frame skipping of exons 6 to 9, whereas the upper band (500pb) represents a non in-frame skipping.