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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: Gene Ther. 2012 May 3;20(3):274–282. doi: 10.1038/gt.2012.38

Figure 8. Purification and sequencing of nested RT PCR product.

Figure 8

(A) The in-frame and the non in-frame skipping band have been extracted from the gel and were sequenced using the inner primer exon 3 and the inner primer exon 10 (purification band #1). (B) Sequences analysis demonstrate that the lower band (305bp) corresponds to skipping of exons 6 to 9 with restoration of open reading frame whereas the upper band (478pb) corresponds to skipping of exons 7 to 9 which did alter the open reading frame.