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. Author manuscript; available in PMC: 2013 Sep 1.
Published in final edited form as: J Immunol. 2012 Jul 27;189(5):2211–2216. doi: 10.4049/jimmunol.1201324

Figure 2. GBV-C E2 protein inhibits T cell receptor (TCR) induced IL-2.

Figure 2

Following TCR activation with anti-CD3/CD28, IL-2 secretion was measured in Jurkat cells expressing GBV-C E2 protein (1–331), the C-terminal (1–219) and N-terminal (220–331) E2 deletions, the E2 coding region with a frame-shift control cell line (FS), or the vector control (VC). IL-2 release was significantly reduced in GBV-C E2 and E2 (1–219) expressing Jurkat cells (A). Jurkat cell IL-2 mRNA levels were measured in the various cell lines following TCR activation (B). Fold increase in IL-2 production was calculated by measuring IL-2 in the culture supernatant before and after activation. IL-2 mRNA levels were measured after activation and normalized to 18S rRNA levels. *P <0.05; **P <0.01; ns = not significant, p>0.05. Each experiment was performed in triplicate using three independent cultures and repeated at least three times on different days with consistent results.