FIG. 1.

The evolution of Puf proteins in fungi. The phylogenetic tree for species in fungi was reconstructed based on ten conserved proteins (the bootstrap scores are displayed in Supplementary fig. 1, Supplementary Material online, and the abbreviations for the studied species are shown in Supplementary table 2, Supplementary Material online). The tree was consistent with the results of a previous study (Fitzpatrick et al. 2006). The color in each cell denotes the number of PUM-HD repeats for that gene in the respective species. Gray means that there was no detectable orthologous gene. The arrows indicate the WGD event and the Saccharomycotina clade, respectively.