FIGURE 2.

Use of Terazosin (α₁AR antagonist) and SB265610 (CXCR2 inverse agonist) to interrogate β-arrestin2 recruitment to the α_1AAR-CXCR2 heteromer. Extended BRET kinetic profiles were generated for the CXCR2/Rluc8, β-arrestin2/Venus and α_1AAR combination in HEK293FT cells by treating with CXCL8 or vehicle (A) or NE or vehicle (B) ~30 min before a second treatment with vehicle, 10 μM Terazosin, and/or 10 μM SB265610. Data are representative of three independent experiments. This research was originally published in Mustafa et al. (2012). Copyright © 2012 the American Society for Biochemistry and Molecular Biology.