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. 2012 May 16;40(15):7247–7256. doi: 10.1093/nar/gks367

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — Dynamic acetylation of histone H3 is conserved in Dictyostelium and preferentially found on H3b. (A–B) Histone extracts from TSA (1 μg/ml, 3.3 μM) treated exponentially growing Ax2 and H3b/c^−/− cells were isolated at various times of treatment up to 4 h and analysed by (A) 18% SDS–PAGE or (B) 15% acetic AU (AU) gel electrophoresis and western blots were performed with anti-H3K4me3, H3K9ac, -H3K14ac and -H3 polyclonal antibodies to show the induction level and distribution. A. Positions of histone H3 variants are shown on the right of each panel. (B) Position of differentially charged H3 species are labelled 0 = presumed unmodified to 3.