Figure 3.

Northern blot of GFP mRNA. Cells were grown in the presence or absence of galactose as indicated. Total RNA was isolated from the indicated primary tov strains, the isogenic wild-type strain containing the GAL-IT-GFP plasmid (DY1514) or isogenic wild-type strain with a GAL-GFP reporter plasmid lacking the IT (DY1513). The agarose gel was blotted to a membrane and the filter was probed with a radio-labeled PCR product containing the GFP reading frame. The 12 lanes on the left were from a single gel and filter. The six lanes on the right were from a different single gel and filter (the origin of the higher mobility, cross-hybridizing band seen in all lanes is unknown).