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. 2012 May 10;40(15):7358–7367. doi: 10.1093/nar/gks407

Figure 2.

Figure 2.

Loss of both BLM and WRN RecQ helicases exacerbates TDs. (A) Images of FISH of metaphase chromosomes from GM02548-E6E7 (BS) fibroblasts after WRN knockdown and AG05229-E6E7 (WS) fibroblasts after BLM knockdown. Arrows indicate chromosomes in the magnified panels below each metaphase spread. (B) Percentage of chromatids with TDs. At least 2000 chromatids from each cell line were counted, and average values were compiled from two independent experiments. (C) IMR90 and WS cells were fixed with 4% PFA and used for IF-FISH with a BLM antibody and the telomeric probe FITC-[CCCTAA]4. IF-FISH images of indicated fibroblast cell lines, showing BLM (red) and telomeres (green); arrows show colocalization events. (D) Average number of BLM foci per cell and (E) percentage of cells with more than two colocalization events between BLM and telomeres. The values for each cell line were averaged from three independent IF-FISH experiments. Arrow bars represent standard deviation, and P-values were calculated by a Student’s t-test (*P < 0.05 and **P < 0.005).