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. 2012 May 14;40(15):7430–7441. doi: 10.1093/nar/gks416

Figure 6.

Figure 6.

High resolution mapping of the cross-link between the primer S-20 21-mer and TER. (A) Mapping of the cross-link site of the primer S-20 21-mer was performed as illustrated in Figure 3B and described in the legend to Figure 5. The band denoted as c contains fragments generated by secondary cleavage of the fragments denoted as ‘a’ by RNase H, as indicated in the drawing shown in Figure 3A-III. Lane 1: no RNase H, no complementary oligo. Lane 2: oligo complementary to the region of TER spanned by the nucleotides 24–40. Lane 3: oligo complementary to the region of TER spanned by the nucleotides 25–41. Lanes 4–11: overlapping oligos of the same series: 26–42, … ,33–49. Lane 12: no oligo. (B) A plot of the data shown in A. (C) RNase H cleavage assay of hybrids formed between the overlapping oligos used for the assays shown in A and uniformly labeled TER molecules synthesized in vitro. (D) A plot of the data shown in C.