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The cryptic promoter in the pMB1 ori within pRF is robust and constitutively active. Shown are levels of cryptic and expected RNA produced from pRF constructs containing (A) or lacking (B) the SV40P. RNA from transfected cells was analysed by quantitative RT-PCR to detect total cryptic RNA, spliced readthrough cryptic RNA, or the expected RNA from the SV40P. Asterisks indicate the absence of detectable transcript. Error bars, SD.
