Figure 4.

Nucleotide variations between H₃₇Rv and H₃₇Ra result in gain or loss of serine/threonine phosphorylation sites. (A) The relative percentage of members of PE/PPE, mce, MmpL, Sigma, TCSS families exhibiting difference in phosphorylation sites between H₃₇Rv and H₃₇Ra is shown. (B, C) Gain of serine/threonine phosphorylation sites. The predictive phosphorylation site(s) in proteins is represented by blue circle (in the case of H₃₇Rv) or red dots (in case of H₃₇Ra). Overlap of blue and red circle indicates no gain or loss of phosphorylation sites in H₃₇Rv and H₃₇Ra proteins. Horizontal line represents the cutoff (threshold) above which it is considered as a putative phosphorylation site. The actual sequence of amino acids and the potential phosphorylation site (highlighted in red) is shown above the respective figures. N-terminal extension in MRA_1801 (B) or MRA_2433 (C) leads to gain of additional serine/threonine phosphorylation sites.