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. 2012 May 24;40(15):7319–7331. doi: 10.1093/nar/gks472

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© The Author(s) 2012. Published by Oxford University Press.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 5. — In the transcriptionally active state of ftz-f1, SAYP is essential for the assembly of Brahma at the point of transient pausing as well as for Ser2 phosphorylation and transcription elongation. (A) Distribution profiles of SAYP, Brahma (PB and Moira) and TFIID (TAF1 and TBP) subunits, Pol II and nucleosomes (α-histone H3 antibody) along the ftz-f1 gene at the (+;–) stage (3 h after ecdysone removal) as determined by ChIP in normal cells (solid line) and after SAYP knockdown (dotted line). (B) Distribution profiles of Pol II Ser5-(P) and Pol II Ser2-(P) along the ftz-f1 gene at the (+;–) stage (3 h after ecdysone removal) in normal cells (solid line) and after SAYP knockdown (dotted line). (C) Changes in the level of ftz-f1 transcription at the (+;–) stage at different time points after ecdysone removal in cells after SAYP RNAi knockdown (light bars) and in normal cells (dark bars (Figure 1C). Transcription was detected with two pairs of primers corresponding to positions 1 and 4 in Figure 1A), which produced the same results.