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. 2012 Jun 21;40(15):e115. doi: 10.1093/nar/gks596

Figure 3.

Figure 3.

Example output web page from Primer3Plus. The first major block, labeled ‘Pair 1’, shows the template sequence with locations of the primers highlighted in blue and yellow and key information about the primers and primer pair. Subsequent blocks (‘Pair 2’,…, ‘Pair 5’) show information for alternative primer pairs.