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. 2012 Jun 21;40(15):e115. doi: 10.1093/nar/gks596

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Published by Oxford University Press 2012.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 4. — Examples of boulder IO (A) input to primer3_core and (B) output from primer3_core. Panel A shows two primer design tasks (SEQUENCE_ID’s example 1 and example 2) separated by a line consisting only of an = character. PRIMER_NUM_RETURN is 1 for both tasks, because the values of tags beginning with PRIMER_… persist between design tasks. However, the value of tags beginning with SEQUENCE_…, such as SEQUENCE_EXCLUDED_REGION = 37,21 in example 1, affect only the current design task. Panel B shows abbreviated output corresponding to panel A.