Skip to main content
. Author manuscript; available in PMC: 2012 Aug 22.
Published in final edited form as: Cell Microbiol. 2009 Feb 22;11(6):898–910. doi: 10.1111/j.1462-5822.2009.01300.x

Figure 4. Myb domain protein overexpression generated expression pattern similar to Entamoeba cysts.

Figure 4

Figure 4

(A) Venn diagram of overlap in transcriptome between N-Myc EhMyb-dr overexpressing trophozoites and E. histolytica cysts. Of the 117 genes with increased expression in EhMyb-dr overexpressing parasites, 40 are also upregulated in E. histolytica cysts (p= 6.5e-18). Of the 88 genes with increased expression in EhMyb-dr overexpressing parasites, 30 are downregulated in E. histolytica cysts (p= 3.0e-12). (B) RT-PCR confirms that genes regulated by N-Myc EhMyb-dr overexpression in E. histolytica trophozoites have encystation-specific homologues in E. invadens. For all samples, a control reaction without reverse transcriptase was performed and was negative. EIN_136300 was a loading control (Ehrenkaufer et al., 2007b).