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. Author manuscript; available in PMC: 2012 Aug 22.
Published in final edited form as: Cell Microbiol. 2009 Feb 22;11(6):898–910. doi: 10.1111/j.1462-5822.2009.01300.x

Figure 6. Electrophoretic mobility shift assays with the M5 motif.

Figure 6

EMSA with a putative EhMyb-dr binding motif, M5 (CCCCCC) in nuclear extract from wild type and N-Myc EhMyb-dr overexpressing parasites. This motif shows a specific band, which can be competed away by specific but not by non-specific competitors and has higher signal from the N-Myc EhMyb-dr parasite nuclear extract. Multiple EMSA experiments were performed and the mean intensity ± standard error are shown. A representative EMSA gel is shown.