Figure 4. Action potentials are evoked in VCN neurons at embryonic ages.

A, APs are first reliably elicited by AN stimulation at E16. For comparison, representative waveforms for ages E15–P0 are aligned (0 mV) at resting membrane potential, with latency relative to AN stimulus (0 ms, arrow). B, AP half-width decreased with age. C, latency from AN stimulation to AP peak decreased with age (n = 6–9 for all ages in panels B and C). Open black circles indicate values for individual neurons, filled red circles indicate average values and error bars show SD.