Figure 2.

An example of autoradiograms for isolation of species specific highly repetitive sequences by array comparative genomic hybridization. The nylon membrane corresponding to the left panel carried different clones picked up from an SSY genomic library, and was hybridized with SSY genomic DNA labeled with alkaline phosphatase as probe. The nylon membrane for the right panel contained the same set of the clones, prepared as a duplicate, and hybridized with genomic DNA of HAG. The upper-left part of the autoradiogram obtained from each membrane is shown here. The signal strength reflects the repetitiveness of each clone: the higher the copy number in the genome, the more intense the signal. The pattern of the signal strength among the clones is roughly the same between the two panels, except for one at the B-4 position. The clone at this position was assumed, and subsequently demonstrated, to be highly repetitive in the SSY genome but not in the HAG genome.