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. 1977 Apr;4(4):899–915. doi: 10.1093/nar/4.4.899

Characterization of mRNA-protein complexes from mammalian cells.

B Sundquist, T Persson, U Lindberg
PMCID: PMC342493  PMID: 866197

Abstract

In a previous report we described the use of oligo(dT)-cellulose for the isolation of mRNA-protein complexes from EDTA-dissociated polysomes extracted from normally growing or adenovirus infected KB-cells (I). Experiments presented here provide evidence that proteins involved in these complexes bind specifically to mRNA since: a) the proteins and mRNA cosediment through sucrose gradients, b) they adsorb and elute from oligo(dT)-cellulose together, and c) analysis of the products from ribonuclease digestion experiments show that the poly (A) end and a separate small fraction of the mRNA are resistant to the enzymes and attached to protein.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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