Expression of LIN28 and LIN28B during human fetal ovarian development. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) analysis reveals expression of LIN28
(A) to be highest at 8–11 wga, and significantly lower at 14–16 wga and 17–20 wga, coincident with the onset of germ cell differentiation [***P<0.001 vs. 8–11 wga (ANOVA with Neumann-Keuls post hoc testing), n=6–8 ovaries per gestational group]. LIN28B expression (B) remained unchanged across gestation. Values denote mean±SEM. ANOVA, analysis of variance; SEM, standard error of the mean.