Skip to main content
PMC home page
Nucleic Acids Research logoLink to Nucleic Acids Research
. 1977 Apr;4(4):1111–1122. doi: 10.1093/nar/4.4.1111

Oligo(dG)12-18 aggregates result in non-homogeneity of oligo(dG)12-18.poly(C) type primer-template.

R Mikke, B Zmudzka
PMCID: PMC342508  PMID: 68464

Abstract

Studies on the absorption spectra of equimolar solutions of oligo(dG)12-18 and poly(C), poly(Cm) or poly(Ce) showed that only 13%, 3% and 3% of base pairs, repectively, form complexes. Upon centrifugation of oligo(dG)12-18 with a molar excess of poly(C) of poly(Cm) in an analytical and a preparative centrifuge, it was found that only a part of oligodeoxynucleotide sediments with the polynucleotide, i.e. more rapidly than oligo(dG)12-18, poly(C) or poly(Cm). Products of binding of oligo(dG)12-18 with poly(C), poly(Cm) or poly (Ce) direct the synthesis of poly (dG) by AMV reverse transcriptas in accordance with the reported characteristics of these primer-templates, as well as of the enzyme. These observations suggest that the solutions of oligo(dG)12-18 with (polyC) or its analogues, commonly used as primer-templates of RNA- and DNA-directed DNA polymerases, contain a polynucleotide, to which oligo(dG)12-18 aggregates are bound through a few nucleotides chains shorter than 12-18 residues. These chains of oligo(dG)12-18 containing the 3'-OH ends are capable of of initiating the reaction with DNA POLYMERASES.

Full text

PDF
1122

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Baltimore D., Smoler D. Primer requirement and template specificity of the DNA polymerase of RNA tumor viruses. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1507–1511. doi: 10.1073/pnas.68.7.1507. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chamberlin M. J. Comparative properties of DNA, RNA, and hybrid homopolymer pairs. Fed Proc. 1965 Nov-Dec;24(6):1446–1457. [PubMed] [Google Scholar]
  3. Gerard G. F., Loewenstein P. M., Green M., Rottman F. Detection of reverse transcriptase in human breast tumours with poly(Cm)-oligo(dG). Nature. 1975 Jul 10;256(5513):140–143. doi: 10.1038/256140a0. [DOI] [PubMed] [Google Scholar]
  4. Gerard G. F. Poly (2'O-methylcytidylate).oligodeoxyguanylate, a template-primer specific for reverse transcriptase, is not utilized by HeLa cell gamma DNA polymerases. Biochem Biophys Res Commun. 1975 Apr 7;63(3):706–711. doi: 10.1016/s0006-291x(75)80441-4. [DOI] [PubMed] [Google Scholar]
  5. Green M., Gerard G. F. RNA-directed DNA polymerase--properties and functions in oncogenic RNA viruses and cells. Prog Nucleic Acid Res Mol Biol. 1974;14(0):187–334. [PubMed] [Google Scholar]
  6. Hurwitz J., Leis J. P. RNA-dependent DNA polymerase activity of RNA tumor viruses. I. Directing influence of DNA in the reaction. J Virol. 1972 Jan;9(1):116–129. doi: 10.1128/jvi.9.1.116-129.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. INMAN R. B., BALDWIN R. L. HELIX--RANDOM COIL TRANSITIONS IN DNA HOMOPOLYMER PAIRS. J Mol Biol. 1964 Apr;8:452–469. doi: 10.1016/s0022-2836(64)80003-6. [DOI] [PubMed] [Google Scholar]
  8. Kacian D. L., Spiegelman S. Purification and detection of reverse transcriptase in viruses and cells. Methods Enzymol. 1974;29:150–173. doi: 10.1016/0076-6879(74)29018-9. [DOI] [PubMed] [Google Scholar]
  9. Kielanowska M., Shugar D. Preparation and properties of poly 2'-O-ethylcytidylic acid. Nucleic Acids Res. 1976 Mar;3(3):817–824. doi: 10.1093/nar/3.3.817. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lefler C. F., Bollum F. J. Deoxynucleotide-polymerizing enzymes of calf thymus gland. 3. Preparation of poly N-acetyldeoxyguanylate and polydeoxyguanylate. J Biol Chem. 1969 Feb 25;244(4):594–601. [PubMed] [Google Scholar]
  11. Mikke R., Kielanowska M., Shugar D., Zmudzka B. Poly 2'-O-ethylcytidylate, an inhibitor and poor template for AMV reverse transcriptase. Nucleic Acids Res. 1976 Jun;3(6):1603–1611. doi: 10.1093/nar/3.6.1603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Riley M., Maling B. Physical and chemical characterization of two- and three-stranded adenine-thymine and adenine-uracil homopolymer complexes. J Mol Biol. 1966 Sep;20(2):359–389. doi: 10.1016/0022-2836(66)90069-6. [DOI] [PubMed] [Google Scholar]
  13. Spiegelman S., Burny A., Das M. R., Keydar J., Schlom J., Trávnícek M., Watson K. Synthetic DNA-RNA hybrids and RNA-RNA duplexes as templates for the polymerases of the oncogenic RNA viruses. Nature. 1970 Oct 31;228(5270):430–432. doi: 10.1038/228430a0. [DOI] [PubMed] [Google Scholar]
  14. Tazawa I., Tazawa S., Ts'o P. O. Studies on oligonucleotides: thermodynamic and optical properties of the oligoinosinate-polycytidylate complexes. J Mol Biol. 1972 Apr 28;66(1):115–130. doi: 10.1016/s0022-2836(72)80010-x. [DOI] [PubMed] [Google Scholar]
  15. Wu A. M., Gallo R. C. Reverse transcriptase. CRC Crit Rev Biochem. 1975 Dec;3(3):289–347. doi: 10.3109/10409237509105454. [DOI] [PubMed] [Google Scholar]
  16. Zmudzka B., Janion C., Shugar D. Poly 2'-O-methylcytidylic acid and the role of the 2'-hydroxyl in polynucleotide structure. Biochem Biophys Res Commun. 1969 Dec 4;37(6):895–901. doi: 10.1016/0006-291x(69)90215-0. [DOI] [PubMed] [Google Scholar]
  17. Zmudzka B., Tichy M., Shugar D. The structure of poly 2'-O-methylcytidylic acid and its complexes with polyinosinic acid. Acta Biochim Pol. 1972;19(2):149–160. [PubMed] [Google Scholar]

Articles from Nucleic Acids Research are provided here courtesy of Oxford University Press

RESOURCES