Table I. Information for glasshouse experiments and field tests evaluating the pollen ablation effect in plants.
The glasshouse experiments and field tests are listed in sequential order as they appear in the text.
| Experiment Materialsa and Establishment Year | Construct Name | Cassette(s) Carried by the Indicated Constructsbc | Experiment Location |
|---|---|---|---|
| Tobacco, 2003 | pWVR234 | PrMC2pro-I-GUS | Summerville, South Carolina |
| Tobacco, 2004 | pAGF245 | PrMC2pro-II-GUS | Summerville, South Carolina |
| Tobacco, 2003 | pWVR220 | PrMC2pro-I-barnaseH102E | Summerville, South Carolina |
| Tobacco, 2003 | pWVR216 | PrMC2pro-I-barnaseK27A | Summerville, South Carolina |
| Tobacco, 2003 | pWVR232 | PrMC2pro-I-barnaseE73G | Summerville, South Carolina |
| Tobacco, 2004 | pAGF243 | PrMC2pro-II-barnaseH102E | Summerville, South Carolina |
| PxL grafts, 2005 | pWVR220 | PrMC2pro-I-barnaseH102E | Southern Georgia |
| PxL grafts, 2005 | pWVR234 | PrMC2pro-I-GUS | Southern Georgia |
| Field-grown PxL trees, 2004 | pWVR220 | PrMC2pro-I-barnaseH102E | Eastern South Carolina |
| Loblolly grafts, 2007 | pWVK310 | PrMC2pro-II-barnaseH102E and PrUBQ-AtGOGAT | Southern Georgia |
| Loblolly grafts, 2007 | pWVK312 | PrMC2pro-II-barnaseH102E and PrUBQ-PsGS | Southern Georgia |
| Loblolly grafts, 2007 | pAGK316 | PrMC2pro-II-barnaseH102E and Pt4CL-PtUDP-GBD | Southern Georgia |
| Loblolly grafts, 2007 | pAGK320 | PrMC2pro-II-barnaseH102E and PrQUB-AtGOGAT and Pt4CL-PtUDP-GBD | Southern Georgia |
| Loblolly grafts, 2007 | pAGK321 | PrMC2pro-II-barnaseH102E and PrUBQ-PsGS and Pt4CL-PtUDP-GBD | Southern Georgia |
| E. occidentalis, 2005 | pARB598 | PrMC2pro-I-barnaseH102E and Pt4CL-Eg4CL antisense | Summerville, South Carolina |
| E. occidentalis, 2006 | pAGF243 | PrMC2pro-II-barnaseH102E | Summerville, South Carolina |
| Field-grown EH1, 2005 | pARB598 | PrMC2pro-I-barnaseH102E and Pt4CL-Eg4CL antisense | Central Florida |
| Field-grown EH1, 2005 | pARB599 | PrMC2pro-I-barnaseH102E and Pt4CL- Eg4CL RNA interference | Central Florida |
| Field-grown EH1, 2005 | pABCTE01 | PrMC2pro-II-barnaseH102E and AtRd29A-AtCBF2 | Southern Alabama |
Tobacco and E. occidentalis were grown in the glasshouse. bPromoter and gene symbols are as follows: PrMC2pro-I, PrMC2 promoter version I from P. radiata; PrMC2pro-II, PrMC2 promoter version II from P. radiata; PrUBQ, polyubiquitin promoter from P. radiata; AtGOGAT, Arabidopsis Glu synthase; PsGS, Gln synthetase from pea (Pisum sativum); Pt4CL, 4-coumarate CoA ligase gene promoter from P. taeda; PtUDP-GBD, the UDP-Glc-binding domain protein gene from Populus tremuloides; Eg4CL, 4-coumarate CoA ligase gene from E. grandis; AtRd29A, Arabidopsis Rd29A gene promoter; AtCBF2, Arabidopsis C-repeat binding factor 2. cEach construct also carries the same selectable marker cassette consisting of the E. coli nptII gene driven by an Arabidopsis ubiquitin promoter.