Figure 3.

Quantity of the Driselase-generated xyloglucan signature fragment, IP, estimated by HPAEC analysis. Results are expressed as relative amounts of IP obtained after digestion of total AIR, and soluble in 1 n KOH and 4 n KOH fractions prepared from mutant lines in comparison with the amount of IP obtained from the same fraction prepared from Col-0 wild-type plants, which was taken as 100%. One milligram of each sample was dissolved in 30 µL of acetate buffer containing approximately 0.5 units of Driselase and incubated for 18 h at 37°C. The total volume of each digest was analyzed by HPAEC. Analyses were performed on three independent sample preparations for each mutant and wild-type plant. Results were averaged and expressed as mean values (n = 3 biological replications) ± sd.