Figure 5.

Glycome profiling of cell wall extracts prepared from Arabidopsis Col-0 and xxt mutants. Cell walls of each plant line were prepared from whole seedlings of Arabidopsis Col-0 wild type (WT) and all single and multiple xxt mutants grown in liquid culture. The walls were subjected to sequential extraction with α-amylase, CDTA, 1 n KOH, and 4 n KOH as described in “Materials and Methods.” The solubilized extracts were then screened against an array of plant glycan-directed monoclonal antibodies (Pattathil et al., 2010) using ELISAs. The panels at right (colored boxes) depict the groups of antibodies used, identified according to the polysaccharides predominantly recognized by each group (for additional details about the individual antibodies used, see Supplemental Table S2). The extent of antibody binding is represented as a colored heat map, with the brightest yellow depicting the strongest binding and black depicting no binding.