Figure 5.

Detection of maize-derived transcripts associated with C₄ photosynthesis in leaves of OMA plants. Total RNA was isolated from mature leaves of OMA plants and resolved on a 1.2% agarose-formaldehyde gel. RNA (10 µg per lane) was hybridized with a radiolabeled maize probe for the transcript of interest. RNA from maize, oat, and an OMA line with a different maize chromosome were probed as controls. rRNA (25S) from the gel used for blotting stained with ethidium bromide is shown as a loading control below each blot. Transcripts detected with maize-specific ZmCA2 (2.0 kb), ZmDCT2 (1.9 kb), ZmMDH6 (1.5 kb), ZmDCT1 (2.1 kb), ZmME1 (2.2 kb), ZmPPDK (3.5 kb), ZmPEPC1 (3.2 kb), and ZmOMT1 (1.9 kb) probes were of the expected sizes.