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Steady-state accumulation and localization of chloroplast proteases. Chloroplasts were purified from mature leaves of Col and var2 using a Percoll step gradient. Intact chloroplasts were fractionated into stroma and membrane fractions. Proteins were separated using SDS-PAGE and probed against specific antibodies. D1 and Rubisco large subunits were used as markers of membranes and stroma, respectively. Samples were equally loaded based on chlorophyll content.
