Figure 5.

Rescue of the saul1 senescence and molecular phenotypes in saul1/pad4 double mutants. A, The phenotypes of wild-type (WT), saul1-1 and pad4 single mutant, and saul1-1/pad4 double mutant plants, which were grown for 4 weeks in permissive light, were studied 3 d after transfer to low light. The saul1 mutants but not the saul1/pad4 double mutants exhibited yellowing of leaves and reduced growth. B, Induction of gene expression of senescence- and SA-related genes. Relative expression levels for AAO3, WRKY6, and EDS16 were determined by qPCR from three independent experiments. Means ± sd (n = 3) are shown for wild-type (light gray bars), pad4 (black bars), saul1-1 (white bars), and saul1-1/pad4 double mutant (dark gray bars) seedlings. Induction of all three genes was only seen in saul1-1 seedlings. This induction was prevented in saul1-1/pad4 double mutants.