Figure 3.

Knockdown mutant srl1-2, by T-DNA insertion, presents similar phenotype of srl1-1. A, The leaves of srl1-2 are also adaxially rolled from seedling to mature stage compared with wild-type plants ZH11. 1, 14-d-old seedlings, bar = 1 cm; 2, 30-d-old plants, bar = 10 cm; 3, mature plants, bar = 10 cm; 4, section of the second leaf from top of mature plants, bar = 1 mm. B, Cross sections of the third leaf blades of 14-d-old ZH11 (left section) and srl1-2 (right section) seedlings show significantly increased bulliform cells between vascular bundles in srl1-2. Bars = 100 μm. C, Toluidine blue O staining of the cells between small vascular bundle and midrib in ZH11 and srl1-2 (the third leaf of 14-d-old seedlings). Bulliform cells and epidermal cells were stained in purple and blue, respectively. Bars = 50 μm.