Figure 2.

Enhanced tolerance to various stresses in nrt1.5 mutants. A, Five-day-old seedlings were transferred to one-half-strength Murashige and Skoog medium (½ × MS) or one-half-strength Murashige and Skoog medium supplemented with 125 mm NaCl or 50 μm CdCl₂ and allowed another 6 d to grow. B, Root elongation between days 2 and 7 after transfer to the treatments in A. Values are means ± sd from three replicates, and each contained eight plants. ** P < 0.01. C, Seed germination rate on one-half-strength Murashige and Skoog medium supplemented with the indicated levels of NaCl. Values are means ± sd (n = 200 seeds). ** P < 0.01. D, Representative photographs for drought tolerance analysis of wild-type Col-0 and nrt1.5 mutant plants. Control (top panel), drought-stressed (middle panel), and rewatered (bottom panel) plants were treated as described in “Materials and Methods” before imaging. E, Water loss rate of leaves detached from 16-d-old plants. Values are means ± sd (n = 15). FW, Fresh weight.