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. 2012 Jun 19;159(4):1591–1607. doi: 10.1104/pp.112.199018

Figure 1.

Figure 1.

NaCDPK4 and NaCDPK5 are expressed in specific tissues and are induced by wounding and simulated herbivore feeding. Constructs harboring the promoters of NaCDPK4 and NaCDPK5 fused with GUS reporter gene were transformed into N. attenuata to create NaCDPK4Pro:GUS and NaCDPK5Pro:GUS plants, respectively. GUS histochemical assays were performed to examine the expression of NaCDPK4 (A) and NaCDPK5 (B) in seedlings (I), leaves (II), wounded leaves (III), stems (IV), flowers (V and VI), young seed capsules (VII), and developing seeds (VIII). N. attenuata was wounded with a pattern wheel and 20 μL of water (W + W) or M. sexta OS (W + OS) were immediately applied to wounds and relative transcript levels (mean ± se) of NaCDPK4 and NaCDPK5 (C) were determined with qRT-PCR in samples harvested at indicated times. Asterisks indicate significant differences between W + W- and W + OS-treated samples (n = 5; Student’s t test; **, P < 0.01; ***, P < 0.001).