Figure 4.

IRcdpk4/5 plants have dramatically increased JA and JA-Ile levels after wounding and simulated herbivory treatment. Wild type (WT) and IRcdpk4/5 (line IRcdpk4/5-1 and IRcdpk4/5-2) were wounded with a pattern wheel, and 20 μL of water (W + W) or M. sexta OS (W + OS) were immediately applied to wounds. Contents (mean ± se) of JA (A) and JA-Ile (B) and relative transcript levels (mean ± se) of genes involved JA biosynthesis (C) were determined in W + OS-treated samples after indicated times. Asterisks indicate significant differences between WT and IRcdpk4/5 plants (n = 5; Student’s t test; *, P < 0.05; **, P < 0.01; ***, P < 0.001).