Figure 5.

NaCDPK4 and NaCDPK5 have redundant roles in controlling herbivory-induced JA levels. Agrobacterium carrying pTV00 (EV), pTV-NaCDPK4, pTV-NaCDPK5, and pTV-NaCDPK4/5 was inoculated into N. attenuata to create EV, VIGS-NaCDPK4, VIGS-NaCDPK5, and VIGS-NaCDPK4/5, respectively. A, Relative transcript levels (mean ± se) of NaCDPK4 and NaCDPK5 in untreated plants. Note: Levels NaCDPK4 and NaCDPK5 in EV are designated to 1. B, JA contents (mean ± se) in plants after simulated herbivore feeding. Plants were wounded with a pattern wheel and 20 μL of M. sexta OS were applied to wounds and samples were collected 1 h after treatment.