Figure 7.

Silencing COI1 in IRcdpk4/5 plants abolishes herbivore defenses. IRcdpk4/5-1 was crossed with IRcoi1 to generate IRcdpk4/5-1 × IRcoi1. A, Masses of M. sexta larvae grown on wild type (WT), IRcdpk4/5-1, IRcoi1, and IRcdpk4/5-1 × IRcoi1. Concentrations (mean ± se) of HGL-DTGs (B) and CP (C), and activity (mean ± se) of TPIs (C) were quantified in plants that had been fed for 12 d. Asterisks indicate significant differences between WT and other plants (n = 30 for A; n = 5 for B, C, and D; t test; *, P < 0.05; **, P < 0.01; ***, P < 0.001). n.d., Not detected.