Figure 9.

Knocking down SIPK or WIPK in IRcdpk4/5 plants decreases wounding- and simulated herbivory-induced JA levels. IRcdpk4/5-1 was crossed with IRwipk and IRsipk to create IRcdpk4/5-1 × IRwipk and IRcdpk4/5-1 × IRsipk, respectively. Plants were wounded with a pattern wheel and 20 μL of water or M. sexta OS were applied to wounds immediately (W + W and W + OS, respectively). Samples were harvested after 30 min and the JA contents (mean ± se) were determined on an HPLC-tandem mass spectrometer (n = 5).