Phenotypic analysis of OsMGT1 knockout lines. A, Sensitivity of OsMGT1 knockout lines to Al. Seedlings of wild-type rice (WT) and two OsMGT1 knockout lines (NF0595 and NE4528) were exposed to a solution containing different concentration of Al (0, 10, 30, and 50 μm) for 24 h. The root length was measured before and after the treatment. Data are means ± sd (n = 10). The asterisk shows a significant difference at P < 0.05 by Tukey’s test. B, Sensitivity of OsMGT1 knockout lines to other metals. WT and two knockout lines were exposed to a solution containing 50 μm
Al, 10 μm
Cd, or 5 μm
La for 24 h and root elongation relative to the root growth without metals is shown. Data are means ± sd (n = 10). The asterisk shows a significant difference (P < 0.05 by Tukey’s test). C, Growth on acidic soil. Germinated seeds were sowed on acidic soil (Andsol, pH 4.5) or neutral soil (pH 6.5) and grown for 1 week.