Figure 7.

²⁵Mg uptake in OsMGT1 knockout lines. A, A time-dependent uptake of ²⁵Mg. Seedlings of wild-type and knockout lines were exposed to a solution containing 10 μm ²⁵Mg. At different time points, the roots and shoots were sampled for determination of ²⁵Mg with ICP-MS. B, Effect of Al on ²⁵Mg uptake. Seedlings of wild-type and knockout lines were pretreated with or without Al (0 or 50 μm, pH 4.5) for 6 h and subsequently subjected to an uptake solution containing different concentrations of ²⁵Mg. After 30 min, the roots were sampled for determination of ²⁵Mg. Data are means ± sd (n = 3). C, OsMGT1-mediated Mg uptake. The net uptake was calculated by subtracting Mg uptake of knockout lines from that of wild-type rice.