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. 2012 Jun 27;159(4):1634–1643. doi: 10.1104/pp.112.199786

Figure 3.

Figure 3.

Functional characterization of GmPT5 in yeast. MB192 is a yeast mutant defective in high-affinity Pi uptake. Yp112-GmPT5 contained GmPT5 in the expression vector p112A1NE (abbreviated Yp112) and transformed into MB192. A, Growth of yeast cells. Equal volumes of 10-fold serial dilution with the original cell number of 6 × 105 were applied to YNB medium supplied with 100 or 50 μm Pi and then incubated at 30°C for 3 d. B, Rate of [33P]Pi transport by Yp112-GmPT5 and Yp112 at different Pi concentrations. Nonlinear regression of Pi uptake of strain Yp112-GmPT5 and Yp112 versus external Pi concentration at pH 6 was used to estimate the Km value.