Figure 4.

Characterization of the β-GlcY precipitate using native electrophoresis methods. A, Rocket gel electrophoresis run on an agarose gel containing β-GlcY reagent. Numbers below the rockets give the amount of gum arabic loaded (mg) and from which the concentration of Yariv-positive material in the root cap fractions was estimated. Bn and Ps indicate material from B. napus and pea, respectively. B, Linear arabinogalactan protein profiles revealed by agarose gel electrophoresis followed by staining with β-GlcY reagent. gA, Twenty micrograms of gum arabic; Rw, 10 µg of red wine arabinogalactan proteins. Different populations of arabinogalactan proteins are indicated by white arrows. C, Two-dimensional arabinogalactan protein profile revealed by cross electrophoresis followed by staining with β-GlcY reagent. Different populations of arabinogalactan proteins are indicated by white arrows. [See online article for color version of this figure.]