Figure 3.

Analysis of in vitro hydrogenase activity (A), HYDA1 transcript (B), and HYDA1 protein (C) levels in crr1 mutant CC-3960 and strain CC-425 upon S deficiency. A, Cells were subjected to S deprivation in sealed flasks in the light. At the indicated time points, cell samples were withdrawn and analyzed by in vitro hydrogenase activity assays. Black triangles: CC-425 (CRR1), white triangles: CC-3960 (crr1-2; Kropat et al., 2005). Average values ± sd of two independent experiments are shown. B, RNA-hybridization analyses using DIG-labeled HYDA1- and RPL10a-specific probes on RNA samples isolated from S-deprived cells after the indicated time points. C, HYDA1 immunoblot analyses using polyclonal anti-C. reinhardtii-HYDA1 antibody on crude protein extracts prepared from S-starved cultures. HYDA1 transcript and HYDA1 protein amounts were analyzed from both strains examined in two independent experiments. One representative result is shown.