Figure 7.

EMSAs analyzing the capacity of the heterologously produced CRR1 SBP domain to bind to HYDA1 promoter fragments. A, Schematic drawing representing the genomic upstream region of the HYDA1 gene as shown in Figure 5. Boxes below this scheme indicate the fragments used for EMSAs in their wild-type form (white boxes) or in a mutated form (gray box; GTAC was exchanged by TAGA). The individual fragments are labeled with Roman numerals, which assign them to the respective EMSA shown in B. B, DIG-labeled fragments I to IV were incubated without (−) or with (+) 200 ng of the recombinant CRR1 SBP domain, separated by native PAGE, and visualized using antidigoxigenin antibody.