Skip to main content
. 2012 Jun 27;159(4):1759–1770. doi: 10.1104/pp.112.200881

Figure 2.

Figure 2.

Characterization of pOpON::amiREXP transgenic plants. A and B, A 12-DAS first leaf pair (A) and an 18-DAS leaf 6 (B) from a pOpON::amiREXP plant treated with (+) or without (−) 10 µm Dex overnight prior to the visualization of GUS activity (blue coloration). C, GUS staining of a 20-DAS pOpON::amiREXP plant grown with 10 µm Dex. D, Micrograph of induced leaf 6 from a 20-DAS pOpON::amiREXP plant showing induction in all cells. E, Expression levels of amiRNA-targeted (EXPA10, -1, -5, -3) and nontargeted (EXPA8, -13, -15) expansin genes in 12-DAS plants grown on medium supplemented with 10 µm Dex (gray bars) or control medium (white bars) determined by real-time qPCR analysis. Values are means ± se from three biological replicates, normalized to the amount of ACT2 and UBC21. F, Comparison of pOpON::amiREXP and wild-type (WT) plants grown with 10 µm Dex or control medium at 31 DAS. Bars = 5 mm (A–C), 100 µm (D), and 5 mm (F). [See online article for color version of this figure.]