Figure 5.

bda1-17D constitutively activates cell death and defense responses. A, Phenotypes of wild-type (WT), bda1-17D, and BDA1/bda1-17D heterozygous plants. Plants were grown on soil and photographed approximately 4 weeks after planting. B and C, Expression of PR1 (B) and PR2 (C) in the wild type and bda1-17D determined by qPCR. RNA was extracted from 25-d-old soil-grown plants. Values were normalized to the expression of ACTIN1. Error bars represent sd from three measurements. D, Free and total SA in the wild type and bda1-17D. Error bars represent sd from four measurements. E, Growth of H.a. Noco2 on the wild type and bda1-17D. Sixteen-day-old seedlings were sprayed with H.a. Noco2 spores (50,000 spores mL⁻¹). Infection was scored 7 d after inoculation. The values presented are averages of three measurements ± sd. fw, Fresh weight. F, Morphology of 4-week-old soil-grown wild-type, bda1-17D, and transgenic lines expressing the bda1-17D mutant gene in the wild-type background. G, Growth of H.a. Noco2 on wild-type and transgenic lines expressing the bda1-17D mutant gene. Sixteen-day-old seedlings were sprayed with H.a. Noco2 spores (50,000 spores mL⁻¹). Infection was scored 7 d after inoculation. The values presented are averages of three measurements ± sd. [See online article for color version of this figure.]